Cell Biology Antibodies 9

NONO/p54nrb Rabbit Polyclonal Antibody (CAB5282)

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SKU:
CAB5282
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114, NONO/p54nrb
Research Use:
Cell Biology
Frequently bought together:

Description

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NONO/p54nrb Antibody (CAB5282)

The NONO/p54nrb Antibody (CAB5282) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the Nono protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential resource for studies in molecular biology, cell biology, and genetics.The Nono protein, also known as non-POU domain-containing octamer-binding protein, plays a critical role in RNA processing, DNA repair, and gene expression regulation.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NONO/p54nrb Antibody
SKU:CAB5282
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MQSN KTFN LEKQ NHTP RKHH QHHH QQQH HQQQ QQQP PPPP IPAN GQQA SSQN EGLT IDLK NFRK PGEK TFTQ RSRL FVGN LPPD ITEE EMRK LFEK YGKA GEVF IHKD KGFG FIRL ETRT LAEI AKVE LDNM PLRG KQLR VRFA CHSA SLTV RNLP QYVS NELL EEAF SVFG QVER AVVI VDDR GRPS GKGI VEFS GKPA ARKA LDRC SEGS FLLT TFPR PVTV EPMD QLDD EEGL PEKL VIKN QQFH KERE QPPR FAQP GSFE YEYA MRWK ALIE MEKQ QQDQ VDRN IKEA REKL EMEM
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114, NONO/p54nrb
Positive Sample:BxPC-3, 293T, Mouse brain
Cellular Localization:Nucleus, Nucleus Speckle, Nucleolus.
Calculated MW:54kDa
Observed MW:58kDa

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Purification MethodAffinity purification
Gene ID4841
RRIDAB_2766100
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using NONO/p54nrb Rabbit pAb (CAB5282) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using NONO/p54nrb Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using NONO/p54nrb Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human thyroid cancer using NONO/p54nrb Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using NONO/p54nrb Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using NONO/p54nrb Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using NONO/p54nrb Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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