The NGB Antibody (CAB6477) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in a variety of applications, including Western blotting and immunohistochemistry.Neuroglobin is a globin protein predominantly found in neurons, where it acts as a cytoprotective molecule against oxidative stress and hypoxic conditions. Studies have shown that neuroglobin can protect against neuronal cell death and promote cell survival in various neurological disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
NGB Antibody
SKU:
CAB6477
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NGB
Positive Sample:
Mouse brain
Cellular Localization:
Cytoplasm, Mitochondrion, Perikaryon.
Calculated MW:
17kDa
Observed MW:
17kDa
This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions.
Purification Method
Affinity purification
Gene ID
58157
RRID
AB_2767078
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using NGB Rabbit pAb (CAB6477) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
