Cell Biology Antibodies 1

Neurofilament L Rabbit Polyclonal Antibody (CAB0257)

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SKU:
CAB0257
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
NFL, NF-L, NF68, CMT1F, CMT2E, CMTDIG, PPP1R110, Neurofilament L
Research Use:
Cell Biology
Frequently bought together:

Description

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Neurofilament L Antibody (CAB0257)

The Neurofilament L Antibody (CAB0257) is a high-quality antibody developed for reliable detection and analysis of target proteins. Neurofilaments are structural proteins found in neurons, and abnormalities in these proteins have been linked to various neurological conditions such as Alzheimer's disease, ALS, and neuropathies.This antibody, developed using rabbits as the host animal, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically targeting the NEFL protein, researchers can detect and analyze neurofilament expression in a variety of cell types, aiding in the investigation of neurodegenerative diseases and potential therapeutic targets.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Neurofilament L Antibody
SKU:CAB0257
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SSGS LMPS LENL DLSQ VAAI SNDL KSIR TQEK AQLQ DLND RFAS FIER VHEL EQQN KVLE AELL VLRQ KHSE PSRF RALY EQEI RDLR LAAE DATN EKQA LQGE REGL EETL RNLQ ARYE EEVL SRED AEGR LMEA RKGA DEAA LARA ELEK RIDS LMDE ISFL KKVH EEEI AELQ AQIQ YAQI SVEM DVT
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NFL, NF-L, NF68, CMT1F, CMT2E, CMTDIG, PPP1R110, Neurofilament L
Positive Sample:293T, SH-SY5Y, U-251MG, Mouse brain, Rat brain
Cellular Localization:Axon, Axon Cytoplasm, Cholinergic Synapse, Cytoplasm, Cytosol, Growth Cone, Neurofilament, Neuromuscular Junction, Postsynaptic Intermediate Filament Cytoskeleton, Presynaptic Intermediate Filament Cytoskeleton, Schaffer Collateral - Ca1 Synapse.
Calculated MW:62kDa
Observed MW:70kDa

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Purification MethodAffinity purification
Gene ID4747
RRIDAB_2757070
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Neurofilament L Rabbit pAb (CAB0257) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using Neurofilament L Rabbit pAb (CAB0257) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using Neurofilament L Rabbit pAb (CAB0257) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded rat brain using Neurofilament L Rabbit pAb (CAB0257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse brain using Neurofilament L Rabbit pAb (CAB0257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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