Cell Biology Antibodies 12

Neurofilament H Rabbit Polyclonal Antibody (CAB8442)

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SKU:
CAB8442
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
NFH, CMT2CC, Neurofilament H
Research Use:
Cell Biology
Frequently bought together:

Description

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Neurofilament H Antibody (CAB8442)

The Neurofilament H Antibody (CAB8442) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting. By specifically binding to NEFH, this antibody enables precise detection and analysis of the protein in different neuronal cell types.NEFH is essential for maintaining the structural integrity of neurons and plays a crucial role in neuronal development and function. Dysregulation of NEFH has been linked to various neurodegenerative diseases, making it a promising target for research in neurology and neuroscience.

This antibody is validated for use in WB, IF/ICC, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Neurofilament H Antibody
SKU:CAB8442
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KVEE KKEP AVEK PKES KVEA KKEE AEDK KKVP TPEK EAPA KVEV KEDA KPKE KTEV AKKE PDDA KAKE PSKP AEKK EAAP EKKD TKEE KAKK PEEK PKTE AKAK EDDK TLSK EPSK PKAE KAEK SSST DQKD SKPP EKAT EDKA AKGK
Tested Applications:WB IF/ICC IP ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IP0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
IF/ICC1:50 - 1:200
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NFH, CMT2CC, Neurofilament H
Positive Sample:Mouse brain, Mouse liver, Rat brain, Rat liver
Cellular Localization:Axon, Cytoplasm, Cytoskeleton, Neurofilament, Postsynaptic Density, Postsynaptic Intermediate Filament Cytoskeleton, Schaffer Collateral - Ca1 Synapse.
Calculated MW:112kDa
Observed MW:150kDa/220kDa

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Purification MethodAffinity purification
Gene ID4744
RRIDAB_2770557
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Neurofilament H Rabbit pAb (CAB8442) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of C6 cells using Neurofilament H Rabbit pAb (CAB8442) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg Neurofilament H antibody (CAB8442). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (CAB8442) at a dilution of 1:1000.
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