The NDUFS3 Monoclonal Antibody (CAB4602) is a high-quality antibody developed for reliable detection and analysis of target proteins. NDUFs3, a subunit of mitochondrial complex I, is crucial in the electron transport chain and ATP production. The antibody, produced in rabbits, is highly specific and reactive with human samples, validated for use in Western blot applications.NDUFs3 is essential for cellular energy production and metabolic function, making it a key player in mitochondrial function and overall cell health. Dysfunction of NDUFs3 is linked to various diseases, including mitochondrial disorders, neurodegenerative diseases, and metabolic syndromes.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
NDUFS3 Monoclonal Antibody
SKU:
CAB4602
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1049
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CI-30, MC1DN8, NDUFS3
Positive Sample:
Hep G2, SH-SY5Y
Cellular Localization:
Mitochondrion Inner Membrane.
Calculated MW:
30kDa
Observed MW:
28kDa
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
Purification Method
Affinity purification
Gene ID
4722
RRID
AB_2863308
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NDUFS3 Rabbit mAb (CAB4602) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using NDUFS3 Rabbit mAb (CAB4602) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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