NDUFB8 Rabbit Polyclonal Antibody (CAB20457)

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SKU:
CAB20457
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
ASHI, CI-ASHI, MC1DN32, NDUFB8
Research Use:
Metabolism
Frequently bought together:

Description

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NDUFB8 Antibody (CAB20457)

The NDUFB8 Antibody (CAB20457) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to NDUFB8, the antibody enables precise detection and analysis of this important protein in various cell types.NDUFB8 is essential for the assembly and function of complex I in the electron transport chain, making it a key player in cellular energy production. Dysregulation of complex I has been implicated in various human diseases, including neurodegenerative disorders and metabolic syndromes.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NDUFB8 Antibody
SKU:CAB20457
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ASHM TKDM FPGP YPRT PEER AAAA KKYN MRVE DYEP YPDD GMGY GDYP KLPD RSQH ERDP WYSW DQPG LRLN WGEP MHWH LDMY NRNR VDTS PTPV SWHV MCMQ LFGF LAFM IFMC WVGD VYPV YQPV GPKQ YPYN NLYL ERGG DPSK EPER VVHY EI
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ASHI, CI-ASHI, MC1DN32, NDUFB8
Positive Sample:U-251MG, HepG2, A-549, Mouse brain, Mouse heart, Mouse kidney, Rat brain, Rat heart, Rat liver
Cellular Localization:Endoplasmic Reticulum, Mitochondrial Inner Membrane, Mitochondrial Matrix, Mitochondrial Respiratory Chain Complex I, Mitochondrion.
Calculated MW:22kDa
Observed MW:20kDa

Involved in mitochondrial respiratory chain complex I assembly. Located in endoplasmic reticulum and mitochondrion. Part of mitochondrial respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency 32. Biomarker of Alzheimer's disease and Parkinson's disease.

Purification MethodAffinity purification
Gene ID4714
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NDUFB8 Rabbit pAb (CAB20457) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver using NDUFB8 Rabbit pAb (CAB20457) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of A-549 cells using NDUFB8 Rabbit pAb (CAB20457) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using NDUFB8 Rabbit pAb (CAB20457) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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