Cell Death Antibodies 2

NDUFA13/GRIM19 Rabbit Polyclonal Antibody (CAB5412)

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SKU:
CAB5412
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
B16.6, CDA016, CGI-39, GRIM19, GRIM-19, MC1DN28, [KD Validated] NDUFA13/GRIM19
Research Use:
Cell Death
Frequently bought together:

Description

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NDUFA13/GRIM19 Antibody (CAB5412)

The NDUFA13/GRIM19 Antibody (CAB5412) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting NDUFA13 in human samples, making it ideal for Western blot applications. By targeting the NDUFA13 protein, researchers can gain insights into mitochondrial function and its potential role in various biological processes.NDUFA13 is a key player in cellular respiration, playing a crucial role in converting nutrients into energy through oxidative phosphorylation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NDUFA13/GRIM19 Antibody
SKU:CAB5412
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAAS KVKQ DMPP PGGY GPID YKRN LPRR GLSG YSML AIGI GTLI YGHW SIMK WNRE RRRL QIED FEAR IALL PLLQ AETD RRTL QMLR ENLE EEAI IMKD VPDW KVGE SVFH TTRW VPPL IGEL YGLR TTEE ALHA SHGF MWYT
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:B16.6, CDA016, CGI-39, GRIM19, GRIM-19, MC1DN28, [KD Validated] NDUFA13/GRIM19
Positive Sample:293T, HepG2, PC-3
Cellular Localization:Matrix Side, Mitochondrion Inner Membrane, Nucleus, Single-Pass Membrane Protein.
Calculated MW:17kDa
Observed MW:17kDa

This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.

Purification MethodAffinity purification
Gene ID51079
RRIDAB_2766220
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and NDUFA13/GRIM19 knockout (KO) HeLa cells, using [KO Validated] NDUFA13/GRIM19 Rabbit pAb (CAB5412) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of various lysates, using [KD Validated] NDUFA13/GRIM19 Rabbit pAb (CAB5412) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from wild type(WT) and NDUFA13/GRIM19 knockdown (KD) 293T cells, using [KD Validated] NDUFA13/GRIM19 Rabbit pAb (CAB5412) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
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