Cell Biology Antibodies 12

NDUFA12 Rabbit Polyclonal Antibody (CAB8237)

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SKU:
CAB8237
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
B17.2, DAP13, MC1DN23, NDUFA12
Research Use:
Cell Biology
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Description

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NDUFA12 Antibody (CAB8237)

The NDUFA12 Antibody (CAB8237) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the NDUFA12 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in areas such as bioenergetics, mitochondrial dysfunction, and metabolic disorders.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NDUFA12 Antibody
SKU:CAB8237
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MELV QVLK RGLQ QITG HGGL RGYL RVFF RTND AKVG TLVG EDKY GNKY YEDN KQFF GRHR WVVY TTEM NGKN TFWD VDGS MVPP EWHR WLHS MTDD PPTT KPLT ARKF IWTN HKFN VTGT PEQY VPYS TTRK KIQE WIPP STPY K
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:B17.2, DAP13, MC1DN23, NDUFA12
Positive Sample:293T, PC-12, HepG2, Mouse kidney, Mouse heart, Mouse testis, Rat heart, Rat brain
Cellular Localization:Matrix Side, Mitochondrion Inner Membrane, Peripheral Membrane Protein.
Calculated MW:17kDa
Observed MW:17kDa

This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID55967
RRIDAB_2770544
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NDUFA12 Rabbit pAb (CAB8237) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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