Antibody Name:	Anti-NCF4 Antibody
Antibody SKU:	CAB2096
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCF4 (NP_038202.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, Jurkat, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCF4 (NP_038202.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVA QQLR AESD FEQL PDDV AISA NIAD IEEK RGFT SHFV FVIE VKTK GGSK YLIY RRYR QFHA LQSK LEER FGPD SKSS ALAC TLPT LPAK VYVG VKQE IAEM RIPA LNAY MKSL LSLP VWVL MDED VRIF FYQS PYDS EQVP QALR RLRP RTRK VKSV SPQG NSVD RMAA PRAE ALFD FTGN SKLE LN
Gene ID:	4689
Uniprot:	Q15080
Cellular Location:	Cytoplasm, Cytoplasmic side, Endosome membrane, Membrane, Peripheral membrane protein, cytosol
Calculated MW:	39kDa
Observed MW:	42kDa

Synonyms:

NCF4, CGD3, NCF, P40PHOX, SH3PXD4

Background:

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed.
UniProt Protein Details:	Protein type:Oxidoreductase Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: cytosol; endosome membrane; membrane; NADPH oxidase complex Molecular Function:phosphatidylinositol 3-phosphate binding; protein binding; protein dimerization activity; superoxide-generating NADPH oxidase activator activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; immune response; vascular endothelial growth factor receptor signaling pathway Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Iii
NCBI Summary:	The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15080
NCBI GenInfo Identifier:	108884815
NCBI Gene ID:	4689
NCBI Accession:	Q15080.2
UniProt Secondary Accession:	Q15080,O60808, Q86U56, Q9BU98, Q9NP45, A8K4F9,
UniProt Related Accession:	Q15080
Molecular Weight:	39,017 Da
NCBI Full Name:	Neutrophil cytosol factor 4
NCBI Synonym Full Names:	neutrophil cytosolic factor 4
NCBI Official Symbol:	NCF4
NCBI Official Synonym Symbols:	NCF; CGD3; P40PHOX; SH3PXD4
NCBI Protein Information:	neutrophil cytosol factor 4
UniProt Protein Name:	Neutrophil cytosol factor 4
UniProt Synonym Protein Names:	Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox
Protein Family:	Neutrophil cytosol factor
UniProt Gene Name:	NCF4
UniProt Entry Name:	NCF4_HUMAN

	Western blot analysis of extracts of various cell lines, using NCF4 antibody (CAB2096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using NCF4 Antibody (CAB2096) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using NCF4 Antibody (CAB2096) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using NCF4 Antibody (CAB2096) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using NCF4 antibody (CAB2096). Blue: DAPI for nuclear staining.