The GALT Monoclonal Antibody (CAB20966) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in various applications, including Western blotting.GALT is essential for converting galactose-1-phosphate into UDP-galactose, a key step in galactose utilization and energy production. Mutations in the GALT gene can lead to galactosemia, a rare genetic disorder that affects the body's ability to metabolize galactose.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GALT Monoclonal Antibody
SKU:
CAB20966
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2955
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GALT
Positive Sample:
HeLa, HepG2
Cellular Localization:
Cytoplasm, Cytosol, Golgi Apparatus.
Calculated MW:
43kDa
Observed MW:
43kDa
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2592
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GALT Rabbit mAb (CAB20966) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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