Cell Biology Antibodies 1

MYH9 Rabbit Polyclonal Antibody (CAB0173)

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SKU:
CAB0173
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
MHA, FTNS, EPSTS, BDPLT6, DFNA17, MATINS, NMMHCA, NMHC-II-A, NMMHC-IIA, MYH9
Research Use:
Cell Biology
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Description

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MYH9 Antibody (CAB0173)

The MYH9 Antibody (CAB0173) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, displays high reactivity with human samples and has been validated for use in Western blot applications.Myosin 9 is a molecular motor protein that plays a critical role in cell movement and division, as well as in the maintenance of cell shape and structure. Dysregulation of myosin 9 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:MYH9 Antibody
SKU:CAB0173
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ANSS GKGA LALE EKRR LEAR IAQL EEEL EEEQ GNTE LIND RLKK ANLQ IDQI NTDL NLER SHAQ KNEN ARQQ LERQ NKEL KVKL QEME GTVK SKYK ASIT ALEA KIAQ LEEQ LDNE TKER QAAC KQVR RTEK KLKD VLLQ VDDE RRNA EQYK DQAD KAST RLKQ LKRQ LEEA EEEA QRAN ASRR KLQR ELED ATET ADAM NREV SSLK NKLR RGDL PFVV PRRM ARKG AGDG SDEE VDGK ADGA EAKP AE
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:1000 - 1:2000
IHC-P1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MHA, FTNS, EPSTS, BDPLT6, DFNA17, MATINS, NMMHCA, NMHC-II-A, NMMHC-IIA, MYH9
Positive Sample:HeLa, Jurkat, LO2, SW480, Mouse liver, Mouse lung
Cellular Localization:Cytoplasm, Cell Cortex, Cytoskeleton.
Calculated MW:227kDa
Observed MW:250kDa

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.

Purification MethodAffinity purification
Gene ID4627
RRIDAB_2756999
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using MYH9 Rabbit pAb (CAB0173) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophagus using MYH9 Rabbit pAb (CAB0173) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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