Metabolism Antibodies 2

MTIF2 Rabbit Polyclonal Antibody (CAB16402)

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SKU:
CAB16402
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
MTIF2
Research Use:
Metabolism
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Description

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MTIF2 Antibody (CAB16402)

The MTIF2 Antibody (CAB16402) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is suitable for Western blot applications. By binding specifically to MTIF2, this antibody allows for the detection and analysis of this protein in various cell types, making it an important asset for research in molecular biology and mitochondrial function.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:MTIF2 Antibody
SKU:CAB16402
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GEEI LEVE SEPR AREV VDWR KYEQ EQEK GQED LKII EEKR KEHK EAHQ KARE KYGH LLWK KRSI LRFL ERKE QIPL KPKE KRER DSNV LSVI IKGD VDGS VEAI LNII DTYD ASHE CELE LVHF GVGD VSAN DVNL AETF DGVI YGFN VNAG NVIQ QSAA KKGV KIKL HKII YRLV EDLQ EELS SRLP CAVE EHPV GEAS ILAT FSVT EGKK KVPV AGCR VQKG QLEK QKKF KLTR NGHV IWKG SLTS LKHH KDDI SIVK TGMD CGLS LDED NMEF QVGD RIVC YEEK QIQA KTSW DPGF
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MTIF2
Positive Sample:LO2, A-431, U-251MG, Mouse heart, Mouse kidney, Mouse liver, Mouse spleen, Rat testis
Cellular Localization:Mitochondrion.
Calculated MW:81kDa
Observed MW:81kDa

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins.

Purification MethodAffinity purification
Gene ID4528
RRIDAB_2770468
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using MTIF2 Rabbit pAb (CAB16402) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using MTIF2 Rabbit pAb (CAB16402) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using MTIF2 Rabbit pAb (CAB16402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of L929 cells using MTIF2 Rabbit pAb (CAB16402) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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