Cell Biology Antibodies 9

MRPS30 Rabbit Polyclonal Antibody (CAB5841)

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SKU:
CAB5841
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
PAP, PDCD9, S30mt, MRP-S30, MRPS30
Research Use:
Cell Biology
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Description

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MRPS30 Antibody (CAB5841)

The MRPS30 Antibody (CAB5841) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the MRPS30 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for studies in mitochondrial biology and related fields.MRPS30 is a key component of the mitochondrial ribosome, playing a crucial role in protein synthesis within the mitochondria. Dysfunction of MRPS30 has been implicated in various diseases, including cancer and mitochondrial disorders.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:MRPS30 Antibody
SKU:CAB5841
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PHNP ALAA AALD YRCP VHFY WVRG EEII PRGH RRGR IDDL RYQI DDKP NNQI RISK QLAE FVPL DYSV PIEI PTIK CKPD KLPL FKRQ YENH IFVG SKTA DPCC YGHT QFHL LPDK LRRE RLLR QNCA DQIE VVFR ANAI ASLF AWTG AQAM YQGF WSEA DVTR PFVS QAVI TDGK YFSF FCYQ LNTL ALTT QADQ NNPR KNIC WGTQ SKPL YETI EDND VKGF NDDV LLQI VHFL LNRP KEEK SQLL EN
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PAP, PDCD9, S30mt, MRP-S30, MRPS30
Positive Sample:SW620, HL-60, HeLa, HepG2
Cellular Localization:Mitochondrion.
Calculated MW:50kDa
Observed MW:54kDa

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available.

Purification MethodAffinity purification
Gene ID10884
RRIDAB_2766591
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using MRPS30 Rabbit pAb (CAB5841) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of U2OS cells using MRPS30 Rabbit pAb (CAB5841). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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