Cell Biology Antibodies 9

MRPL28 Rabbit Polyclonal Antibody (CAB5897)

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SKU:
CAB5897
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Rat
Synonyms:
p15, MAAT1, MRPL28
Research Use:
Cell Biology
Frequently bought together:

Description

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MRPL28 Antibody (CAB5897)

The MRPL28 Antibody (CAB5897) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the MRPL28 protein, this antibody enables the detection and analysis of MRPL28 in various cell types, making it suitable for research in the fields of mitochondrial biology and cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:MRPL28 Antibody
SKU:CAB5897
Size:20μL, 100μL
Reactivity:Human, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MPLH KYPV WLWK RLQL REGI CSRL PGHY LRSL EEER TPTP VHYR PHGA KFKI NPKN GQRE RVED VPIP IYFP PESQ RGLW GGEG WILG QIYA NNDK LSKR LKKV WKPQ LFER EFYS EILD KKFT VTVT MRTL DLID EAYG LDFY ILKT PKED LCSK FGMD LKRG MLLR LARQ DPQL HPED PERR AAIY DKYK EFAI PEEE AEWV GLTL EEAI EKQR LLEE KDPV PLFK IYVA ELIQ QLQQ QALS EPAV VQKR ASGQ
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:p15, MAAT1, MRPL28
Positive Sample:MCF7, HepG2, 293T, A-549
Cellular Localization:Mitochondrion.
Calculated MW:30kDa
Observed MW:30kDa

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

Purification MethodAffinity purification
Gene ID10573
RRIDAB_2766645
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using MRPL28 Rabbit pAb (CAB5897) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat ovary using MRPL28 Rabbit pAb (CAB5897) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human gastric cancer using MRPL28 Rabbit pAb (CAB5897) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of U2OS cells using MRPL28 Rabbit pAb (CAB5897). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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