Immunology Antibodies 1

TRIM25 Rabbit Polyclonal Antibody (CAB12938)

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SKU:
CAB12938
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
EFP, Z147, RNF147, ZNF147, TRIM25
Research Use:
Immunology
Frequently bought together:

Description

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TRIM25 Antibody (CAB12938)

The TRIM25 Antibody (CAB12938) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it an ideal choice for Western blotting applications.TRIM25, also known as tripartite motif-containing protein 25, plays a crucial role in the innate immune response by functioning as an E3 ubiquitin ligase and regulating the activation of the RIG-I signaling pathway. By targeting viral RNA and promoting its degradation, TRIM25 contributes to the antiviral defense mechanisms within the host.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:TRIM25 Antibody
SKU:CAB12938
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SLSQ ASAD LEAT LRHK LTVM YSQI NGAS RALD DVRN RQQD VRMT ANRK VEQL QQEY TEMK ALLD ASET TSTR KIKE EEKR VNSK FDTI YQIL LKKK SEIQ TLKE EIEQ SLTK RDEF EFLE KASK LRGI STKP VYIP EVEL NHKL IKGI HQST IDLK NELK QCIG RLQE PTPS SGDP GEHD PAST HKST RP
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EFP, Z147, RNF147, ZNF147, TRIM25
Positive Sample:HeLa, 293T, MCF7
Cellular Localization:Cytoplasm.
Calculated MW:71kDa
Observed MW:72kDa

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.

Purification MethodAffinity purification
Gene ID7706
RRIDAB_2736877
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using TRIM25 Rabbit pAb (CAB12938) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human kidney using TRIM25 Rabbit pAb (CAB12938) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using TRIM25 Rabbit pAb (CAB12938) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of A-431 cells using TRIM25 Rabbit pAb (CAB12938) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg TRIM25 antibody (CAB12938). Western blot was performed from the immunoprecipitate using TRIM25 antibody (CAB12938) at a dilution of 1:1000.
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