Cell Death Antibodies 1

MEK7 Rabbit Polyclonal Antibody (CAB2186)

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SKU:
CAB2186
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
MEK, MKK7, JNKK2, MEK 7, MAPKK7, PRKMK7, SAPKK4, SAPKK-4, K7
Research Use:
Cell Death
Frequently bought together:

Description

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MEK7 Antibody (CAB2186)

The MEK7 Antibody (CAB2186) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.MAP2K7, also known as MEK7, is involved in mediating cellular responses to environmental stimuli and stressors, making it a crucial protein in understanding signaling pathways and potential therapeutic targets.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:MEK7 Antibody
SKU:CAB2186
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAAS SLEQ KLSR LEAK LKQE NREA RRRI DLNL DISP QRPR PTLQ LPLA NDGG SRSP SSES SPQH PTPP ARPR HMLG LPST LFTP RSME SIEI DQKL QEIM KQTG YLTI GGQR YQAE INDL ENLG EMGS GTCG QVWK MRFR KTGH VIAV KQMR RSGN KEEN KRIL MDLD VVLK SHDC PYIV QCFG TFIT NTDV FIAM ELMG TCAE KLKK RMQG PIPE RILG KMTV AIVK ALYY LKEK HGVI HRDV KPSN IL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MEK, MKK7, JNKK2, MEK 7, MAPKK7, PRKMK7, SAPKK4, SAPKK-4, K7
Positive Sample:293T, HeLa, HL-60, Jurkat, PC-3, K-562, Mouse liver, Mouse testis, Mouse brain
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:47kDa
Observed MW:48kDa

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID5609
RRIDAB_2764204
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] MEK7 Rabbit pAb (CAB2186) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageWestern blot analysis of lysates from wild type (WT) and MEK7 knockout (KO) 293T cells, using [KO Validated] MEK7 Rabbit pAb (CAB2186) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunofluorescence analysis of C6 cells using [KO Validated] MEK7 Rabbit pAb (CAB2186) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of L929 cells using [KO Validated] MEK7 Rabbit pAb (CAB2186) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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