Metabolism Antibodies 2

IREB1 Rabbit Polyclonal Antibody (CAB7867)

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SKU:
CAB7867
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1, Aconitase 1 (ACO1)
Research Use:
Metabolism
Frequently bought together:

Description

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IREB1 Antibody (CAB7867)

The IREB1 Antibody (CAB7867) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and compatible with human samples for use in Western blot applications. By binding to the ACO1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in biochemistry, metabolism, and diseases related to iron dysregulation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:IREB1 Antibody
SKU:CAB7867
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSNP FAHL AEPL DPVQ PGKK FFNL NKLE DSRY GRLP FSIR VLLE AAIR NCDE FLVK KQDI ENIL HWNV TQHK NIEV PFKP ARVI LQDF TGVP AVVD FAAM RDAV KKLG GDPE KINP VCPA DLVI DHSI QVDF NRRA DSLQ KNQD LEFE RNRE RFEF LKWG SQAF HNMR II
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1, Aconitase 1 (ACO1)
Positive Sample:HCT116, Mouse kidney, Rat heart, Rat kidney
Cellular Localization:Cytoplasm.
Calculated MW:98kDa
Observed MW:98kDa

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants

Purification MethodAffinity purification
Gene ID48
RRIDAB_2768219
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Aconitase 1 (ACO1) Rabbit pAb (CAB7867) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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