Immunology Antibodies 2

IQGAP1 Rabbit Polyclonal Antibody (CAB5679)

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SKU:
CAB5679
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
SAR1, p195, HUMORFA01, IQGAP1
Research Use:
Immunology
Frequently bought together:

Description

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IQGAP1 Antibody (CAB5679)

The IQGAP1 Antibody (CAB5679) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting IQGAP1 in human samples, making it ideal for use in Western blot applications. By binding to IQGAP1, researchers can analyze its expression and function in various cell types, providing insights into its role in cell signaling pathways and its potential implications in cancer and other diseases.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:IQGAP1 Antibody
SKU:CAB5679
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ELMK LKAQ AHAE NNEF ITWN DIQA CVDH VNLV VQEE HERI LAIG LINE ALDE GDAQ KTLQ ALQI PAAK LEGV LAEV AQHY QDTL IRAK REKA QEIQ DESA VLWL DEIQ GGIW QSNK DTQE AQKF ALGI FAIN EAVE SGDV GKTL SALR SPDV GLYG VIPE CGET YHSD LAEA KKKK LAVG DNNS KWVK HWVK GGYY YYHN LETQ EGGW DEPP NFVQ NSMQ LSRE EIQS SISG VTAA YNRE QLWL ANEG LIT
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SAR1, p195, HUMORFA01, IQGAP1
Positive Sample:A-549, 293T, HepG2, HT-29, HeLa, 293F
Cellular Localization:Cell Membrane.
Calculated MW:189kDa
Observed MW:189kDa/189kDa

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Purification MethodAffinity purification
Gene ID8826
RRIDAB_2766439
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using IQGAP1 Rabbit pAb (CAB5679) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using IQGAP1 Rabbit pAb (CAB5679) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse lung using IQGAP1 Rabbit pAb (CAB5679) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using IQGAP1 Rabbit pAb (CAB5679) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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