Cell Death Antibodies 1

HTRA2 Rabbit Polyclonal Antibody (CAB14877)

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SKU:
CAB14877
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
OMI, MGCA8, PARK13, PRSS25, HTRA2
Research Use:
Cell Death
Frequently bought together:

Description

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HTRA2 Antibody (CAB14877)

The HTRA2 Antibody (CAB14877) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit hosts, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications.HtrA2, also known as high temperature requirement protein A2, is a mitochondrial serine protease that plays a crucial role in regulating cell death and survival. Dysregulation of HtrA2 has been linked to various diseases, including neurodegenerative disorders and cancer.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HTRA2 Antibody
SKU:CAB14877
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PSDR LREF LHRG EKKN SSSG ISGS QRRY IGVM MLTL SPSI LAEL QLRE PSFP DVQH GVLI HKVI LGSP AHRA GLRP GDVI LAIG EQMV QNAE DVYE AVRT QSQL AVQI RRGR ETLT LYVT PEVT E
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:OMI, MGCA8, PARK13, PRSS25, HTRA2
Positive Sample:MCF7
Cellular Localization:Mitochondrion Intermembrane Space, Mitochondrion Membrane, Single-Pass Membrane Protein.
Calculated MW:49kDa
Observed MW:36kDa

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID27429
RRIDAB_2761757
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from MCF7 cells, using HTRA2 Rabbit pAb (CAB14877) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg HTRA2 antibody (CAB14877). Western blot was performed from the immunoprecipitate using HTRA2 antibody (CAB14877) at a dilution of 1:1000.
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