Cell Biology Antibodies 7

HNRNPD Rabbit Polyclonal Antibody (CAB1828)

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SKU:
CAB1828
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
P37, AUF1, AUF1A, HNRPD, hnRNPD0, HNRNPD
Research Use:
Cell Biology
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Description

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HNRNPD Antibody (CAB1828)

The HNRNPD Antibody (CAB1828) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot and immunohistochemistry applications. By binding to the HNRNPD protein, researchers can detect and analyze its expression in different cell types, making it an essential tool for studies in molecular biology and cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HNRNPD Antibody
SKU:CAB1828
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSEE QFGG DGAA AAAT AAVG GSAG EQEG AMVA ATQG AAAA AGSG AGTG GGTA SGGT EGGS AESE GAKI DASK NEED EGHS NSSP RHSE AATA QREE WKMF IGGL SWDT TKKD LKDY FSKF GEVV DCTL KLDP ITGR SRGF GFVL FKES ESVD KVMD QKEH KLNG KVID PKRA KAMK TKEP VKKI FVGG LSPD TPEE KIRE YFGG FGEV ESIE LPMD NKTN KRRG FCFI TFKE EEPV KKIM EKKY HNVG LSKC EIKV AMSK EQYQ QQQQ WGSR GGFA GRAR GRGG DQQS GYGK VSRR GGHQ NSYK PY
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P37, AUF1, AUF1A, HNRPD, hnRNPD0, HNRNPD
Positive Sample:SW620, Jurkat, 293T, HepG2, HeLa, A-549
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:38kDa
Observed MW:43kDa

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Purification MethodAffinity purification
Gene ID3184
RRIDAB_2763865
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HNRNPD Rabbit pAb (CAB1828) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using HNRNPD Rabbit pAb (CAB1828) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse pancreas using HNRNPD Rabbit pAb (CAB1828) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of U2OS cells using HNRNPD Rabbit pAb (CAB1828).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
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