Cell Biology Antibodies 15

HIF3A Rabbit Polyclonal Antibody (CAB20020)

(No reviews yet) Write a Review
SKU:
CAB20020
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
IPAS, MOP7, PASD7, HIF-3A, bHLHe17, HIF3-alpha-1, HIF3A
Research Use:
Cell Biology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

HIF3A Antibody (CAB20020)

The HIF3A Antibody (CAB20020) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for Western blot analysis and other immunological techniques.HIF3A is a key regulator of cellular responses to hypoxia, playing a role in various physiological and pathological processes such as angiogenesis, metabolism, and cancer progression. By targeting HIF3A, researchers can gain insights into these processes and potentially develop new strategies for treating hypoxia-related diseases.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HIF3A Antibody
SKU:CAB20020
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MALG LQRA RSTT ELRK EKSR DAAR SRRS QETE VLYQ LAHT LPFA RGVS AHLD KASI MRLT ISYL RMHR LCAA GEWN QVGA GGEP LDAC YLKA LEGF VMVL TAEG DMAY LSEN VSKH LGLS QLEL IGHS IF
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:IPAS, MOP7, PASD7, HIF-3A, bHLHe17, HIF3-alpha-1, HIF3A
Positive Sample:293F-HIF3A-FlagN
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:72kDa
Observed MW:85kDa

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene.

Purification MethodAffinity purification
Gene ID64344
RRIDAB_2862923
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from 293F-HIF3A-FlagN cells, using HIF3A Rabbit pAb (CAB20020) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of C6 cells using HIF3A Rabbit pAb (CAB20020) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products