Cell Biology Antibodies 10

GRASP65 Rabbit Polyclonal Antibody (CAB6609)

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SKU:
CAB6609
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Synonyms:
P65, GOLPH5, GRASP65
Research Use:
Cell Biology
Frequently bought together:

Description

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GRASP65 Antibody (CAB6609)

The GRASP65 Antibody (CAB6609) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to GORASP1, this antibody allows for the detection and analysis of this protein in various cell types, making it an essential tool for studies in cell biology and molecular biology.GORASP1 is a critical player in Golgi structure and function, influencing processes such as protein trafficking and secretion.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:GRASP65 Antibody
SKU:CAB6609
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ALPL GAPP PDAL PPGP TPED SPSL ETGS RQSD YMEA LLQA PGSS MEDP LPGP GSPS HSAP DPDG LPHF METP LQPP PPVQ RVMD PGFL DVSG ISLL DNSN ASVW PSLP SSTE LTTT AVST SGPE DICS SSSS HERG GEAT WSGS EFEV SFLD SPGA QAQA DHLP QLTL PDSL TSAA SPED GLSA ELLE AQAE EEPA STEG LDTG TEAE GLDS QAQI STTE
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:10 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P65, GOLPH5, GRASP65
Positive Sample:BT474, U-937
Cellular Localization:Cytoplasmic Side, Golgi Apparatus, Peripheral Membrane Protein, Cis-Golgi Network Membrane.
Calculated MW:46kDa
Observed MW:65kDa

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.

Purification MethodAffinity purification
Gene ID64689
RRIDAB_2767199
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using GRASP65 Rabbit pAb (CAB6609) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of A549 cells using GRASP65 Rabbit pAb (CAB6609). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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