Developmental Biology

GAP43 Rabbit Polyclonal Antibody (CAB16857)

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SKU:
CAB16857
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
Reactivity:
Mouse, Rat
Synonyms:
B-50, PP46, GAP-43, GAP43
Research Use:
Developmental Biology
Frequently bought together:

Description

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GAP43 Antibody (CAB16857)

The GAP43 Antibody (CAB16857) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot, immunofluorescence, and immunohistochemistry.GAP43, also known as Growth-Associated Protein 43, is a key player in neuronal plasticity and axonal growth. Its expression is upregulated in response to nerve damage, making it a marker for neuroregeneration.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.

Product Name:GAP43 Antibody
SKU:CAB16857
Size:20μL, 100μL
Reactivity:Mouse, Rat
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MLCC MRRT KQVE KNDD DQKI EQDG IKPE DKAH KAAT KIQA SFRG HITR KKLK GEKK DDVQ AAEA EANK KDEA PVAD GVEK KGEG TTTA EAAP ATGS KPDE PGKA GETP SEEK KGEG DAAT EQAA PQAP ASSE EKAG SAET ESAT KAST DNSP SSKA EDAP AKEE PKQA DVPA AVTA AAAT TPAA EDAA AKAT AQPP TETG ESSQ AEEN IEAV DETK PKES ARQD EGKE EEPE ADQE HA
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:B-50, PP46, GAP-43, GAP43
Positive Sample:mouse brain, rat brain
Cellular Localization:Cell Junction, Cell Membrane, Cell Projection, Cytoplasmic Side, Peripheral Membrane Protein, Filopodium Membrane, Growth Cone Membrane, Synapse.
Calculated MW:25kDa
Observed MW:Refertofigures

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID2596
RRIDAB_2769569
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using GAP43 Rabbit pAb (CAB16857) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using GAP43 Rabbit pAb (CAB16857) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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