Cell Biology Antibodies 7

Filamin A Rabbit Polyclonal Antibody (CAB16376)

(No reviews yet) Write a Review
SKU:
CAB16376
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
FLN, FMD, MNS, OPD, ABPX, CSBS, CVD1, FGS2, FLN1, NHBP, OPD1, OPD2, XLVD, XMVD, FLN-A, ABP-280, Filamin A
Research Use:
Cell Biology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Filamin A Antibody (CAB16376)

The Filamin A Antibody (CAB16376) is a high-quality antibody developed for reliable detection and analysis of target proteins. Filamin A (FLNA) is an actin-binding protein that crosslinks actin filaments and links the cytoskeleton to membrane glycoproteins, playing a key role in cytoskeletal remodeling, cell shape changes, and cell migration. Defects in this gene cause several developmental syndromes including periventricular nodular heterotopia and frontometaphyseal dysplasia.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Filamin A Antibody
SKU:CAB16376
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:NQPA SFAV SLNG AKGA IDAK VHSP SGAL EECY VTEI DQDK YAVR FIPR ENGV YLID VKFN GTHI PGSP FKIR VGEP GHGG DPGL VSAY GAGL EGGV TGNP AEFV VNTS NAGA GALS VTID GPSK VKMD CQEC PEGY RVTY TPMA PGSY LISI KYGG PYHI GGSP FKAK VTGP RLVS NHSL HETS SVFV DSLT KATC APQH GAPG PGPA DASK VVAK GLGL SKAY VGQK SSFT VDCS KAGN NMLL VGVH GPRT PCEE ILVK HVGS RLYS VSYL LKDK GEYT LVVK WGDE HIPG SPYR VVVP
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FLN, FMD, MNS, OPD, ABPX, CSBS, CVD1, FGS2, FLN1, NHBP, OPD1, OPD2, XLVD, XMVD, FLN-A, ABP-280, Filamin A
Positive Sample:Jurkat, HeLa, NIH/3T3, 293T, U-251MG, Mouse lung
Cellular Localization:Cytoplasm, Cell Cortex, Cytoskeleton.
Calculated MW:281kDa
Observed MW:300kDa

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID2316
RRIDAB_2769486
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Filamin A Rabbit pAb (CAB16376) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat heart using Filamin A Rabbit pAb (CAB16376) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse spleen using Filamin A Rabbit pAb (CAB16376) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of HeLa cells using Filamin A Rabbit pAb (CAB16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using Filamin A Rabbit pAb (CAB16376) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products

Filamin A Monoclonal Antibody

Filamin A Monoclonal Antibody

Filamin A Monoclonal AntibodyThe product page for this antibody is being prepared. If you have a question please don't hesitate to get in touch!