Cell Biology Antibodies 12
Anti-Exportin-2 Antibody (CAB9510)
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- SKU:
- CAB9510
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Exportin-2 Antibody |
| Antibody SKU: | CAB9510 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CSE1L (NP_001243064.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | B cells, U-87MG, LO2, BT-474 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CSE1L (NP_001243064.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LEKS QDNV IKVC ASVT FKNY IKRN WRIV EDEP NKIC EADR VAIK ANIV HLML SSPE QIQK QLSD AISI IGRE DFPQ KWPD LLTE MVNR FQSG DFHV INGV L |
| Gene ID: | 1434 |
| Uniprot: | P55060 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 22kDa/103kDa/107kDa/110kDa |
| Observed MW: | 110kDa |
| Synonyms: | CSE1L, CAS, CSE1, XPO2 |
| Background: | Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | Exportin-2: Export receptor for importin-alpha. Mediates importin- alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the XPO2/CSE1 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear export; Karyopherin; Nuclear import Chromosomal Location of Human Ortholog: 20q13 Cellular Component: cytoplasm; cytosol; membrane; nuclear envelope; nucleoplasm; nucleus Molecular Function:nuclear export signal receptor activity; protein binding Biological Process: apoptosis; cell proliferation; protein import into nucleus |
| NCBI Summary: | Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P55060 |
| NCBI GenInfo Identifier: | 62297557 |
| NCBI Gene ID: | 1434 |
| NCBI Accession: | P55060.3 |
| UniProt Secondary Accession: | P55060,O75432, Q32M40, Q9H5B7, Q9NTS0, Q9UP98, Q9UP99 Q9UPA0, A3RLL6, B2R5T4, E1P5Y0, F8W904, |
| UniProt Related Accession: | P55060 |
| Molecular Weight: | 103,879 Da |
| NCBI Full Name: | Exportin-2 |
| NCBI Synonym Full Names: | chromosome segregation 1 like |
| NCBI Official Symbol: | CSE1L |
| NCBI Official Synonym Symbols: | CAS; CSE1; XPO2 |
| NCBI Protein Information: | exportin-2 |
| UniProt Protein Name: | Exportin-2 |
| UniProt Synonym Protein Names: | Cellular apoptosis susceptibility protein; Chromosome segregation 1-like protein; Importin-alpha re-exporter |
| UniProt Gene Name: | CSE1L |
| UniProt Entry Name: | XPO2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CSE1L antibody (CAB9510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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