Immunology Antibodies 1

ELANE Rabbit Polyclonal Antibody (CAB13015)

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SKU:
CAB13015
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
GE, NE, HLE, HNE, ELA2, SCN1, PMN-E, Neutrophil Elastase (ELANE)
Research Use:
Immunology
Frequently bought together:

Description

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ELANE Antibody (CAB13015)

The ELANE Antibody (CAB13015) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.Elane is involved in the process of neutrophil extracellular trap (NET) formation, where neutrophils release their DNA to trap and kill pathogens. Dysregulation of Elane activity has been linked to various inflammatory conditions, making it a promising target for research in immunology, infectious diseases, and autoimmune disorders.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ELANE Antibody
SKU:CAB13015
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:IVGG RRAR PHAW PFMV SLQL RGGH FCGA TLIA PNFV MSAA HCVA NVNV RAVR VVLG AHNL SRRE PTRQ VFAV QRIF ENGY DPVN LLND IVIL QLNG SATI NANV QVAQ LPAQ GRRL GNGV QCLA MGWG LLGR NRGI ASVL QELN VTVV TSLC RRSN VCTL VRGR QAGV CFGD SGSP LVCN GLIH GIAS FVRG GCAS GLYP DAFA PVAQ FVNW IDSI IQRS EDNP CPHP RDPD PASR TH
Tested Applications:WB IHC-P IF/ICC ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
IF-P1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GE, NE, HLE, HNE, ELA2, SCN1, PMN-E, Neutrophil Elastase (ELANE)
Positive Sample:THP-1
Cellular Localization:Cell Surface, Cytoplasm, Cytosol, Extracellular Exosome, Extracellular Region, Extracellular Space, Phagocytic Vesicle.
Calculated MW:29kDa
Observed MW:35kDa

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Purification MethodAffinity purification
Gene ID1991
RRIDAB_2759862
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from THP-1 cells, using Neutrophil Elastase (ELANE) Rabbit pAb (CAB13015) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Neutrophil Elastase (ELANE) Rabbit pAb (CAB13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (CAB13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (CAB13015) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of THP-1 cells using Neutrophil Elastase (ELANE) Rabbit pAb (CAB13015) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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