Metabolism Antibodies 2

EEF1A2 Rabbit Polyclonal Antibody (CAB7327)

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SKU:
CAB7327
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
HS1, STN, EF1A, STNL, DEE33, MRD38, EEF1AL, EIEE33, EF-1-alpha-2, EEF1A2
Research Use:
Metabolism
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Description

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EEF1A2 Antibody (CAB7327)

The EEF1A2 Antibody (CAB7327) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By targeting the EEF1A2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and cancer research.EEF1A2 is essential for protein synthesis and plays a crucial role in cell growth and survival. Dysregulation of EEF1A2 has been implicated in various diseases, including cancer, making it a promising target for therapeutic interventions.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:EEF1A2 Antibody
SKU:CAB7327
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:NPAT VPFV PISG WHGD NMLE PSPN MPWF KGWK VERK EGNA SGVS LLEA LDTI LPPT RPTD KPLR LPLQ DVYK IGGI GTVP VGRV ETGI LRPG MVVT FAPV NITT EVKS VEMH HEAL SEAL PGDN VGFN VKNV SVKD IRRG NVCG DSKS DPPQ EAAQ FTSQ VIIL NHPG QISA GYSP VIDC HTAH IACK FAEL KEKI DRRS GKKL EDNP KSLK SGDA AIVE MVPG KPMC VESF SQYP PLGR FAVR DMRQ TVAV GVIK NVEK KSGG AGKV TKSA QKAQ KAGK
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HS1, STN, EF1A, STNL, DEE33, MRD38, EEF1AL, EIEE33, EF-1-alpha-2, EEF1A2
Positive Sample:MCF7, Mouse heart, Rat skeletal muscle, Rat heart
Cellular Localization:Nucleus.
Calculated MW:50kDa
Observed MW:50kDa

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer.

Purification MethodAffinity purification
Gene ID1917
RRIDAB_2767864
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using EEF1A2 Rabbit pAb (CAB7327) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain tissue using EEF1A2 Rabbit pAb (CAB7327) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human brain tissue using EEF1A2 Rabbit pAb (CAB7327) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using EEF1A2 Rabbit pAb (CAB7327) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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