Cell Biology Antibodies 1

EEA1 Rabbit Polyclonal Antibody (CAB0592)

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SKU:
CAB0592
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
MST105, ZFYVE2, MSTP105, EEA1
Research Use:
Cell Biology
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Description

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EEA1 Antibody (CAB0592)

The EEA1 Antibody (CAB0592) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to the Early Endosome Antigen 1 (EEA1) protein and has been rigorously validated for use in various applications, including immunofluorescence and immunohistochemistry.EEA1 is a key component of the early endosome compartment, playing a crucial role in sorting and trafficking of endocytic vesicles. Its involvement in membrane fusion events and signaling cascades makes it a target of interest in studies of cellular transport processes and intracellular trafficking pathways. The EEA1 Polyclonal Antibody enables precise detection and visualization of EEA1 protein in different cell types and tissues, providing valuable insights into endosomal dynamics and organelle interactions.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:EEA1 Antibody
SKU:CAB0592
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LKAA VEQE KRNQ QILK DQVK KEEE ELKK EFIE KEAK LHSE IKEK EVGM KKHE ENEA KLTM QITA LNEN LGTV KKEW QSSQ RRVS ELEK QTDD LRGE IAVL EATV QNNQ DERR ALLE RCLK GEGE IEKL QTKV LELQ RKLD NTTA AVQE LGRE NQSL QIKH TQAL NRKW AEDN EVQN CMAC GKGF SVTV RRHH CRQC GNIF CAEC SAKN ALTP SSKK PVRV CDAC FNDL QG
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MST105, ZFYVE2, MSTP105, EEA1
Positive Sample:U-87MG, HT-1080, LO2, Mouse kidney, Mouse lung, Rat brain
Cellular Localization:Cytoplasm, Early Endosome Membrane, Peripheral Membrane Protein.
Calculated MW:162kDa
Observed MW:175kDa

Enables 1-phosphatidylinositol binding activity; GTP-dependent protein binding activity; and protein homodimerization activity. Involved in endocytosis; vesicle fusion; and viral RNA genome replication. Located in cytosol and early endosome. Is extrinsic component of plasma membrane.

Purification MethodAffinity purification
Gene ID8411
RRIDAB_2757284
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using EEA1 Rabbit pAb (CAB0592) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using EEA1 Rabbit pAb (CAB0592) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using EEA1 Rabbit pAb (CAB0592) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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