Metabolism Antibodies 1

DLAT Rabbit Polyclonal Antibody (CAB14530)

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SKU:
CAB14530
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
E2, PBC, DLTA, PDCE2, PDC-E2, DLAT
Research Use:
Metabolism
Frequently bought together:

Description

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DLAT Antibody (CAB14530)

The DLAT Antibody (CAB14530) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, offers high reactivity with human samples and has been validated for use in Western blot applications. By targeting the DLAT protein, this antibody enables the detection and analysis of DLAT in various cell types, making it an essential tool for researchers in the fields of metabolism and biochemistry.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DLAT Antibody
SKU:CAB14530
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SLPP HQKV PLPS LSPT MQAG TIAR WEKK EGDK INEG DLIA EVET DKAT VGFE SLEE CYMA KILV AEGT RDVP IGAI ICIT VGKP EDIE AFKN YTLD SSAA PTPQ AAPA PTPA ATAS PPTP SAQA PGSS YPPH MQVL LPAL SPTM TMGT VQRW EKKV GEKL SEGD LLAE IETD KATI GFEV QEEG
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:E2, PBC, DLTA, PDCE2, PDC-E2, DLAT
Positive Sample:K562, HeLa, MCF7
Cellular Localization:Mitochondrion Matrix.
Calculated MW:69kDa
Observed MW:69kDa

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

Purification MethodAffinity purification
Gene ID1737
RRIDAB_2761407
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using DLAT Rabbit pAb (CAB14530) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver tissue using DLAT Rabbit pAb (CAB14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil tissue using DLAT Rabbit pAb (CAB14530) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of Hep G2 cells using DLAT Rabbit pAb (CAB14530) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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