Cell Biology Antibodies 3

DKC1 Rabbit Polyclonal Antibody (CAB12914)

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SKU:
CAB12914
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
DKC, CBF5, DKCX, NAP57, NOLA4, XAP101, DKC1
Research Use:
Cell Biology
Frequently bought together:

Description

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DKC1 Antibody (CAB12914)

The DKC1 Antibody (CAB12914) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.DKC1, also known as dyskerin, plays a crucial role in maintaining telomere length and integrity, which is essential for cell survival and proliferation. Additionally, DKC1 is involved in ribosomal RNA processing, impacting protein synthesis within the cell.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:DKC1 Antibody
SKU:CAB12914
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MADA EVII LPKK HKKK KERK SLPE EDVA EIQH AEEF LIKP ESKV AKLD TSQW PLLL KNFD KLNV RTTH YTPL ACGS NPLK REIG DYIR TGFI NLDK PSNP SSHE VVAW IRRI LRVE KTGH SGTL DPKV TGCL IVCI ERAT RLVK SQQS AGKE YVGI VRLH NAIE GGTQ LSRA LETL TGAL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DKC, CBF5, DKCX, NAP57, NOLA4, XAP101, DKC1
Positive Sample:HeLa, 22Rv1, HepG2, MCF7, K-562
Cellular Localization:Cajal Body, Cytoplasm, Nucleus, Nucleolus.
Calculated MW:58kDa
Observed MW:57kDa

This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID1736
RRIDAB_2759759
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DKC1 Rabbit pAb (CAB12914) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using DKC1 Rabbit pAb (CAB12914) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using DKC1 Rabbit pAb (CAB12914) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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