Cell Death Antibodies 1

DFFB Rabbit Polyclonal Antibody (CAB10110)

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SKU:
CAB10110
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse
Synonyms:
CAD, CPAN, DFF2, DFF40, DFF-40, DFFB
Research Use:
Cell Death
Frequently bought together:

Description

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DFFB Antibody (CAB10110)

The DFFB Antibody (CAB10110) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been extensively validated for use in Western blot applications. By recognizing and binding to the DFFB protein, this antibody enables the detection and analysis of DFFB expression in various cell types, making it an essential asset for studies in cell biology, cancer research, and drug development.DFFB is a crucial component of the caspase-activated DNase (CAD) complex, which is responsible for the fragmentation of DNA during apoptosis.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:DFFB Antibody
SKU:CAB10110
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:DEQA PQRQ RLLA DLLH NVSQ NIAA ETRA EDPP WFEG LESR FQSK SGYL RYSC ESRI RSYL REVS SYPS TVGA EAQE EFLR VLGS MCQR LRSM QYNG SYFD RGAK GGSR LCTP EGWF SCQG PFDM DSCL SRHS INPY SNRE SRIL FSTW NLDH IIEK KRTI IPTL VEAI KEQD GREV DWEY FYGL LFTS ENLK LVHI VCHK KTTH KLNC DPSR IYKP QTRL KRKQ PVRK RQ
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:200 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CAD, CPAN, DFF2, DFF40, DFF-40, DFFB
Positive Sample:A-549, K-562, SW620, Mouse spleen, Mouse thymus
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:39kDa
Observed MW:39kDa

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.

Purification MethodAffinity purification
Gene ID1677
RRIDAB_2757634
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DFFB Rabbit pAb (CAB10110) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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