Cell Biology Antibodies 9

CYB5A Rabbit Polyclonal Antibody (CAB5401)

(No reviews yet) Write a Review
SKU:
CAB5401
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
CYB5, MCB5, METAG, CYB5A
Research Use:
Cell Biology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

CYB5A Antibody (CAB5401)

The CYB5A Antibody (CAB5401) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Cytochrome B5 is a crucial component of the electron transport chain in cells, playing a key role in metabolic processes such as drug metabolism, fatty acid desaturation, and steroid biosynthesis. Dysregulation of cytochrome B5 has been linked to various diseases, including metabolic disorders and cancer.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CYB5A Antibody
SKU:CAB5401
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAEQ SDEA VKYY TLEE IQKH NHSK STWL ILHH KVYD LTKF LEEH PGGE EVLR EQAG GDAT ENFE DVGH STDA REMS KTFI IGEL HPDD RPKL NKPP ETLI
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CYB5, MCB5, METAG, CYB5A
Positive Sample:MCF7, A-549, HT-29, 293T, M21
Cellular Localization:Cytoplasm, Cytoplasmic Side, Endoplasmic Reticulum Membrane, Microsome Membrane, Single-Pass Membrane Protein.
Calculated MW:15kDa
Observed MW:17kDa

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID1528
RRIDAB_2766210
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CYB5A Rabbit pAb (CAB5401) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 120s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using CYB5A Rabbit pAb (CAB5401) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using CYB5A Rabbit pAb (CAB5401) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products