Metabolism Antibodies 2

Cystathionase/CTH Rabbit Polyclonal Antibody (CAB6121)

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SKU:
CAB6121
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
CGL, CSE, Cystathionase/CTH
Research Use:
Metabolism
Frequently bought together:

Description

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Cystathionase/CTH Antibody (CAB6121)

The Cystathionase/CTH Antibody (CAB6121) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CTH protein, this antibody enables the detection and analysis of CTH in various cell types, making it ideal for studies in biochemistry and molecular biology.CTH, also known as cystathionine gamma-lyase, plays a crucial role in the transsulfuration pathway and the production of hydrogen sulfide, an important signaling molecule in the body.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Cystathionase/CTH Antibody
SKU:CAB6121
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MQEK DASS QGFL PHFQ HFAT QAIH VGQD PEQW TSRA VVPP ISLS TTFK QGAP GQHS GFEY SRSG NPTR NCLE KAVA ALDG AKYC LAFA SGLA ATVT ITHL LKAG DQII CMDD VYGG TNRY FRQV ASEF GLKI SFVD CSKI KLLE AAIT PETK LVWI ETPT NPTQ KVID IEGC AHIV HKHG DIIL VVDN TFMS PYFQ RPLA LGAD ISMY SATK YMNG HSDV VMGL VSVN CESL HNRL RFLQ NSLG AVPS PIDC YLCN RGLK TLHV RMEK HFKN GMAV AQFL ESNP WVEK VIYP GLPS HPQH ELVK RQCT GCTG MVTF YIKG TLQH AEIF LKNL KLFT LAES LGGF ESLA ELPA IMTH ASVL KNDR DVLG ISDT LIRL SVGL EDEE DLLE DLDQ ALKA AHPP SGSH S
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CGL, CSE, Cystathionase/CTH
Positive Sample:K-562, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Cellular Localization:Cytoplasm.
Calculated MW:45kDa
Observed MW:45kDa

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID1491
RRIDAB_2766754
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using Cystathionase/CTH Rabbit pAb (CAB6121) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb (CAB6121) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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