Epigenetics & Nuclear Signaling Antibodies 2

CNOT1 Rabbit Polyclonal Antibody (CAB5969)

(No reviews yet) Write a Review
SKU:
CAB5969
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
NOT1, CDC39, HPE12, NOT1H, VIBOS, AD-005, CNOT1
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

CNOT1 Antibody (CAB5969)

The CNOT1 Antibody (CAB5969) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting CNOT1 in human samples, making it suitable for use in Western blot applications.CNOT1 is essential for controlling gene expression by regulating mRNA stability and turnover, playing a crucial role in processes such as cell proliferation, differentiation, and development. Dysregulation of CNOT1 has been linked to various diseases, including cancer and neurological disorders, making it a promising target for therapeutic interventions and biomarker discovery.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:CNOT1 Antibody
SKU:CAB5969
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PRNM RLPD PFTP NLKV DMLS EINI APRI LTNF TGVM PPQF KKDL DSYL KTRS PVTF LSDL RSNL QVSN EPGN RYNL QLIN ALVL YVGT QAIA HIHN KGST PSMS TITH SAHM DIFQ NLAV DLDT EGRY LFLN AIAN QLRY PNSH THYF SCTM LYLF AEAN TEAI QEQI TRVL LERL IVNR PHPW GLLI TFIE LIKN PAFK FWNH EFVH CAPE IEKL FQSV AQCC MGQK QAQQ VMEG TGAS
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:200 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NOT1, CDC39, HPE12, NOT1H, VIBOS, AD-005, CNOT1
Positive Sample:U-87MG, 293T, LO2, A-549, HeLa, mouse brain
Cellular Localization:Cytoplasm, Nucleus, P-Body.
Calculated MW:267kDa
Observed MW:267kDa

Enables armadillo repeat domain binding activity; molecular adaptor activity; and nuclear receptor binding activity. Contributes to poly(A)-specific ribonuclease activity. Involved in several processes, including negative regulation of signal transduction; positive regulation of cytoplasmic mRNA processing body assembly; and regulation of gene expression. Located in P-body and cytosol. Part of CCR4-NOT complex. Implicated in holoprosencephaly.

Purification MethodAffinity purification
Gene ID23019
RRIDAB_2766691
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using CNOT1 Rabbit pAb (CAB5969) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunofluorescence analysis of L929 cells using CNOT1 Rabbit pAb (CAB5969) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products