Signal Transduction Antibodies 3

CLIC4 Rabbit Polyclonal Antibody (CAB7088)

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SKU:
CAB7088
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
H1, huH1, p64H1, CLIC4L, MTCLIC, CLIC4
Research Use:
Signal Transduction
Frequently bought together:

Description

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CLIC4 Antibody (CAB7088)

The CLIC4 Antibody (CAB7088) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples. By targeting the Clic4 protein, researchers can accurately detect and analyze its expression in various cell types, making it ideal for studies in cancer biology and metastasis.Clic4, also known as chloride intracellular channel protein 4, is involved in controlling cell adhesion, motility, and invasion, making it a potential therapeutic target for inhibiting cancer progression and metastasis.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CLIC4 Antibody
SKU:CAB7088
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MALS MPLN GLKE EDKE PLIE LFVK AGSD GESI GNCP FSQR LFMI LWLK GVVF SVTT VDLK RKPA DLQN LAPG THPP FITF NSEV KTDV NKIE EFLE EVLC PPKY LKLS PKHP ESNT AGMD IFAK FSAY IKNS RPEA NEAL ERGL LKTL QKLD EYLN SPLP DEID ENSM EDIK FSTR KFLD GNEM TLAD CNLL PKLH IVKV VAKK YRNF DIPK EMTG IWRY LTNA YSRD EFTN TCPS DKEV EIAY SDVA KRLT K
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:100 - 1:500
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:H1, huH1, p64H1, CLIC4L, MTCLIC, CLIC4
Positive Sample:SW620, Mouse kidney, Rat brain, Rat kidney
Cellular Localization:Cell Junction, Cell Membrane, Cytoplasm, Cytoplasmic Vesicle Membrane, Mitochondrion, Nucleus Matrix, Single-Pass Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:29kDa
Observed MW:28kDa

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

Purification MethodAffinity purification
Gene ID25932
RRIDAB_2767643
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CLIC4 Rabbit pAb (CAB7088) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophageal cancer using CLIC4 Rabbit pAb (CAB7088) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using CLIC4 Rabbit pAb (CAB7088) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of A-549 cells using CLIC4 Rabbit pAb (CAB7088). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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