Cell Biology Antibodies 13

CA5B Rabbit Polyclonal Antibody (CAB17633)

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SKU:
CAB17633
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
CAVB, CA-VB, CA5B
Research Use:
Cell Biology
Frequently bought together:

Description

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CA5B Antibody (CAB17633)

The CA5B Antibody (CAB17633) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.CA5B plays a key role in catalyzing the reversible hydration of carbon dioxide to bicarbonate, which is essential for maintaining acid-base balance in cells. Its dysregulation has been implicated in various diseases, including metabolic disorders and cancer.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CA5B Antibody
SKU:CAB17633
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MVVM NSLR VILQ ASPG KLLW RKFQ IPRF MPAR PCSL YTCT YKTR NRAL HPLW ESVD LVPG GDRQ SPIN IRWR DSVY DPGL KPLT ISYD PATC LHVW NNGY SFLV EFED STDK SVIK GGPL EHNY RLKQ FHFH WGAI DAWG SEHT VDSK CFPA ELHL VHWN AVRF ENFE DAAL EENG LAVI GVFL KLGK HHKE LQKL VDTL PSIK HKDA LVEF GSFD PSCL MPTC PDYW TYSG SLTT PPLS ESVT WIIK KQPV EVDH DQLE QFRT LLFT SEGE KEKR MVDN FRPL QPLM NRTV RSSF RHDY VLNV QAKP KPAT SQAT P
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CAVB, CA-VB, CA5B
Positive Sample:Mouse kidney, Rat kidney, Rat lung
Cellular Localization:Mitochondrion.
Calculated MW:36kDa
Observed MW:32kDa

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes carbonic anhydrase 5B. CA5B, and the related CA5A gene, has its expression localized in the mitochondria though CA5B has a wider tissue distribution than CA5A, which is restricted to the liver, kidneys, and skeletal muscle. A carbonic anhydrase pseudogene (CA5BP1) is adjacent to the CA5B gene and these two loci produce CA5BP1-CA5B readthrough transcripts.

Purification MethodAffinity purification
Gene ID11238
RRIDAB_2768668
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using CA5B Rabbit pAb (CAB17633) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of L929 cells using CA5B Rabbit pAb (CAB17633) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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