Cell Death Antibodies 2

BFAR Rabbit Polyclonal Antibody (CAB8308)

(No reviews yet) Write a Review
SKU:
CAB8308
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
BAR, RNF47, BFAR
Research Use:
Cell Death
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

BFAR Antibody (CAB8308)

The BFAR Antibody (CAB8308) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits a high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the BFAR protein, researchers can effectively detect and analyze its expression in different cell types, making it ideal for studies in cell biology and cancer research.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:BFAR Antibody
SKU:CAB8308
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MEEP QKSY VNTM DLER DEPL KSTG PQIS VSEF SCHC CYDI LVNP TTLN CGHS FCRH CLAL WWAS SKKT ECPE CREK WEGF PKVS ILLR DAIE KLFP DAIR LRFE DIQQ NNDI VQSL AAFQ KYGN DQIP LAPN TGRA NQQM
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BAR, RNF47, BFAR
Positive Sample:HepG2, SKOV3, A-549, SH-SY5Y, SW480, Mouse brain, Mouse heart, Mouse liver, Rat heart
Cellular Localization:Endoplasmic Reticulum Membrane, Multi-Pass Membrane Protein.
Calculated MW:53kDa
Observed MW:53kDa

Enables caspase binding activity; protein-macromolecule adaptor activity; and ubiquitin protein ligase activity. Involved in negative regulation of IRE1-mediated unfolded protein response; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein ubiquitination. Acts upstream of or within negative regulation of apoptotic process. Located in endoplasmic reticulum and membrane.

Purification MethodAffinity purification
Gene ID51283
RRIDAB_2768594
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using BFAR Rabbit pAb (CAB8308) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of C6 cells using BFAR Rabbit pAb (CAB8308) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using BFAR Rabbit pAb (CAB8308) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products