Metabolism Antibodies 1

ATP5C1 Rabbit Polyclonal Antibody (CAB15257)

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SKU:
CAB15257
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
ATP5C, ATP5C1, ATP5CL1
Research Use:
Metabolism
Frequently bought together:

Description

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ATP5C1 Antibody (CAB15257)

The ATP5C1 Antibody (CAB15257) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the ATP5C1 protein, researchers can accurately detect and analyze its expression in various cell types, facilitating studies in bioenergetics, metabolism, and mitochondrial dysfunction.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATP5C1 Antibody
SKU:CAB15257
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ATLK DITR RLKS IKNI QKIT KSMK MVAA AKYA RAER ELKP ARIY GLGS LALY EKAD IKGP EDKK KHLL IGVS SDRG LCGA IHSS IAKQ MKSE VATL TAAG KEVM LVGI GDKI RGIL YRTH SDQF LVAF KEVG RKPP TFGD ASVI ALEL LNSG YEFD EGSI IFNK FRSV ISYK TEEK PIFS LNTV ASAD SMSI YDDI DADV LQNY QEYN LANI IYYS LKES TTSE QSAR MTAM DNAS KNAS EMID KLTL TFNR TRQA VITK ELIE IISG AAAL D
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:200 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ATP5C, ATP5C1, ATP5CL1
Positive Sample:Mouse heart, Mouse brain
Cellular Localization:Mitochondrion, Mitochondrion Inner Membrane, Peripheral Membrane Protein.
Calculated MW:33kDa
Observed MW:35kDa

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.

Purification MethodAffinity purification
Gene ID509
RRIDAB_2762155
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using ATP5C1 Rabbit pAb (CAB15257) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat ovary using ATP5C1 Rabbit pAb (CAB15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using ATP5C1 Rabbit pAb (CAB15257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using ATP5C1 Rabbit pAb (CAB15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using ATP5C1 Rabbit pAb (CAB15257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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