Metabolism Antibodies 2

ATPB Rabbit Polyclonal Antibody (CAB5769)

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SKU:
CAB5769
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
ATP5B, ATPMB, ATPSB, HUMOP2, HEL-S-271, ATPB
Research Use:
Metabolism
Frequently bought together:

Description

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ATPB Antibody (CAB5769)

The ATPB Antibody (CAB5769) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and is validated for use in Western blotting applications. By binding to ATP5B, it allows for the accurate detection and analysis of this protein in a variety of cell types, making it ideal for investigations in bioenergetics, metabolism, and mitochondrial function.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATPB Antibody
SKU:CAB5769
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:YSVF AGVG ERTR EGND LYHE MIES GVIN LKDA TSKV ALVY GQMN EPPG ARAR VALT GLTV AEYF RDQE GQDV LLFI DNIF RFTQ AGSE VSAL LGRI PSAV GYQP TLAT DMGT MQER ITTT KKGS ITSV QAIY VPAD DLTD PAPA TTFA HLDA TTVL SRAI AELG IYPA VDPL DSTS RIMD PNIV GSEH YDVA RGVQ KILQ DYKS LQDI IAIL GMDE LSEE DKLT VSRA RKIQ RFLS QPFQ VAEV FTGH MGKL VPLK ETIK GFQQ ILAG EYDH LPEQ AFYM VGPI EEAV AKAD KLAE EHSS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:10000 - 1:30000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ATP5B, ATPMB, ATPSB, HUMOP2, HEL-S-271, ATPB
Positive Sample:HeLa, RAW 264.7, PC-12
Cellular Localization:Mitochondrion, Mitochondrion Inner Membrane.
Calculated MW:57kDa
Observed MW:52kDa

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Purification MethodAffinity purification
Gene ID506
RRIDAB_2766523
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ATPB Rabbit pAb (CAB5769) at 1:25000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human kidney using ATPB Rabbit pAb (CAB5769) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse intestin using ATPB Rabbit pAb (CAB5769) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using ATPB Rabbit pAb (CAB5769) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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