Cell Biology Antibodies 12

ATP1B2 Rabbit Polyclonal Antibody (CAB9928)

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SKU:
CAB9928
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
AMOG, ATP1B2
Research Use:
Cell Biology
Frequently bought together:

Description

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ATP1B2 Antibody (CAB9928)

The ATP1B2 Antibody (CAB9928) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in applications such as Western blotting.ATP1B2 plays a crucial role in cellular functions such as ion transport, cell signaling, and maintaining cell volume. Dysregulation of ATP1B2 has been linked to various diseases, including neurological disorders, cardiovascular diseases, and cancer.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATP1B2 Antibody
SKU:CAB9928
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:DHTP KYQD RLAT PGLM IRPK TENL DVIV NVSD TESW DQHV QKLN KFLE PYND SIQA QKND VCRP GRYY EQPD NGVL NYPK RACQ FNRT QLGN CSGI GDST HYGY STGQ PCVF IKMN RVIN FYAG ANQS MNVT CAGK RDED AENL GNFV MFPA NGNI DLMY FPYY GKKF HVNY TQPL VAVK FLNV TPNV EVNV ECRI NAAN IATD DERD KFAG RVAF KLRI NKT
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AMOG, ATP1B2
Positive Sample:HepG2, NCI-H460, SH-SY5Y
Cellular Localization:Cell Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:33kDa
Observed MW:42kDa

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID482
RRIDAB_2768501
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ATP1B2 Rabbit pAb (CAB9928) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
Product ImageImmunofluorescence analysis of C6 cells using ATP1B2 Rabbit pAb (CAB9928) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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