Epigenetics & Nuclear Signaling Antibodies 2

ATF1 Rabbit Polyclonal Antibody (CAB5791)

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SKU:
CAB5791
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
TREB36, EWS-ATF1, FUS/ATF-1, ATF1
Research Use:
Epigenetics and Nuclear Signaling
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Description

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ATF1 Antibody (CAB5791)

The ATF1 Antibody (CAB5791) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific for ATF1 in human samples, making it ideal for Western blot applications. By binding to ATF1, this antibody enables researchers to detect and analyze ATF1 protein levels in various cell types, facilitating studies in cancer biology and gene regulation.ATF1 plays a crucial role in controlling the expression of genes involved in cell growth, differentiation, and survival, making it a key player in cellular processes like proliferation and apoptosis.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:ATF1 Antibody
SKU:CAB5791
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MEDS HKST TSET APQP GSAV QGAH ISHI AQQV SSLS ESEE SQDS SDSI GSSQ KAHG ILAR RPSY RKIL KDLS SEDT RGRK GDGE NSGV SAAV TSMS VPTP IYQT SSGQ YIAI APNG ALQL ASPG TDGV QGLQ TLTM TNSG STQQ GTTI LQYA QTSD GQQI LVPS NQVV VQTA SGDM QTYQ IRTT PSAT SLPQ TVVM TSPV TLTS QTTK TD
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:TREB36, EWS-ATF1, FUS/ATF-1, ATF1
Positive Sample:HeLa
Cellular Localization:Nucleus.
Calculated MW:29kDa
Observed MW:36kDa

This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6.

Purification MethodAffinity purification
Gene ID466
RRIDAB_2766543
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using ATF1 Rabbit pAb (CAB5791) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg ATF1 antibody (CAB5791). Western blot was performed from the immunoprecipitate using ATF1 antibody (CAB5791) at a dilution of 1:1000.
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