Metabolism Antibodies 2

ASS1 Rabbit Polyclonal Antibody (CAB2012)

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SKU:
CAB2012
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
ASS, CTLN1, ASS1
Research Use:
Metabolism
Frequently bought together:

Description

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ASS1 Antibody (CAB2012)

The ASS1 Antibody (CAB2012) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards ASS1 in human samples, making it an ideal choice for Western blot applications. By targeting the ASS1 protein, this antibody enables accurate detection and analysis in various cell types, facilitating research in fields such as metabolism, cancer biology, and personalized medicine.ASS1, also known as argininosuccinate synthase 1, plays a key role in the urea cycle by catalyzing the formation of argininosuccinate from citrulline and aspartate.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ASS1 Antibody
SKU:CAB2012
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSSK GSVV LAYS GGLD TSCI LVWL KEQG YDVI AYLA NIGQ KEDF EEAR KKAL KLGA KKVF IEDV SREF VEEF IWPA IQSS ALYE DRYL LGTS LARP CIAR KQVE IAQR EGAK YVSH GATG KGND QVRF ELSC YSLA PQIK VIAP WRMP EFYN RFKG RNDL MEYA KQHG IPIP VTPK NPWS MDEN LMHI SYEA GILE NPKN QAPP GLYT KTQD PAKA PNTP DILE IEFK KGVP VKVT NVKD GTTH QTSL ELFM YLNE VAGK HGVG RIDI VENR FIGM KSRG IYET PAGT ILYH AHLD IEAF TMDR EVRK IKQG LGLK FAEL VYTG FWHS PECE FVRH CIAK SQER VEGK VQVS VLKG QVYI LGRE SPLS LYNE ELVS MNVQ GDYE PTDA TGFI NINS LRLK EYHR LQSK VTAK
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ASS, CTLN1, ASS1
Positive Sample:LO2, HeLa, Mouse heart, Mouse kidney, Rat kidney
Cellular Localization:Cell Body Fiber, Cytoplasm, Cytosol, Endoplasmic Reticulum, Extracellular Exosome, Lysosome, Mitochondrial Outer Membrane, Nucleoplasm.
Calculated MW:47kDa
Observed MW:46kDa

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.

Purification MethodAffinity purification
Gene ID445
RRIDAB_2764036
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using ASS1 Rabbit pAb (CAB2012) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of lysates from HeLa cells, using ASS1 Rabbit pAb (CAB2012) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using ASS1 Rabbit pAb (CAB2012) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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