Cell Biology Antibodies 9

AP2A2 Rabbit Polyclonal Antibody (CAB5391)

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SKU:
CAB5391
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
HIP9, HYPJ, ADTAB, HIP-9, CLAPA2, Alpha2-adaptin/AP2A2
Research Use:
Cell Biology
Frequently bought together:

Description

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AP2A2 Antibody (CAB5391)

The AP2A2 Antibody (CAB5391) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific for human samples, making it an ideal choice for Western blot experiments.AP2A2 is a key player in the process of internalizing cell surface receptors and other molecules, regulating important cellular functions such as signaling and nutrient uptake. By targeting the AP2A2 protein, researchers can gain insights into the mechanisms of endocytosis and its role in various physiological processes.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:AP2A2 Antibody
SKU:CAB5391
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:STDI LATV LEEM PPFP ERES SILA KLKK KKGP STVT DLED TKRD RSVD VNGG PEPA PAST SAVS TPSP SADL LGLG AAPP APAG PPPS SGGS GLLV DVFS DSAS VVAP LAPG SEDN FARF VCKN NGVL FENQ LLQI GLKS EFRQ NLGR MFIF YGNK TSTQ FLNF TPTL ICSD DLQP NLNL QTKP VDPT VEGG AQVQ QVVN IECV SDFT EAPV LNIQ FRYG GTFQ NVSV QLPI TLNK FFQP TEMA SQDF FQRW KQLS NPQQ EVQN IFKA KHPM DTEV TKAK IIGF GSAL LEEV DPNP ANFV GAGI IHTK TTQI GCLL RLEP NLQA QMYR LTLR TSKE AVSQ RLCE LLSA QF
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HIP9, HYPJ, ADTAB, HIP-9, CLAPA2, Alpha2-adaptin/AP2A2
Positive Sample:SH-SY5Y, Mouse liver, Rat liver
Cellular Localization:Cell Membrane, Cytoplasmic Side, Membrane, Peripheral Membrane Protein, Coated Pit.
Calculated MW:104kDa
Observed MW:104kDa

The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.

Purification MethodAffinity purification
Gene ID161
RRIDAB_2766200
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Alpha2-adaptin/AP2A2 Rabbit pAb (CAB5391) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of lysates from SH-SY5Y cells, using Alpha2-adaptin/AP2A2 Rabbit pAb (CAB5391) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of HeLa cells using Alpha2-adaptin/AP2A2 Rabbit pAb (CAB5391) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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