Immunology Antibodies 2

THOC4/ALYREF Rabbit Polyclonal Antibody (CAB6010)

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SKU:
CAB6010
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
ALY, BEF, REF, THOC4, ALY/REF, THOC4/ALYREF
Research Use:
Immunology
Frequently bought together:

Description

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THOC4/ALYREF Antibody (CAB6010)

The THOC4/ALYREF Antibody (CAB6010) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the ALYREF protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in molecular biology and gene expression research.ALYREF plays a crucial role in RNA processing, including mRNA export and splicing, making it a key player in gene expression regulation.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:THOC4/ALYREF Antibody
SKU:CAB6010
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MPDS APAM ADKM DMSL DDII KLNR SQRG GRGG GRGR GRAG SQGG RGGG AQAA ARVN RGGG PIRN RPAI ARGA AGGG GRNR PAPY SRPK QLPD KWQH DLFD
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ALY, BEF, REF, THOC4, ALY/REF, THOC4/ALYREF
Positive Sample:HeLa, A-549, B cells, SKOV3, HL-60, Mouse testis, Mouse spleen, Mouse thymus, Rat brain
Cellular Localization:Cytoplasm, Nucleus, Nucleus Speckle.
Calculated MW:27kDa
Observed MW:30kDa

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

Purification MethodAffinity purification
Gene ID10189
RRIDAB_2766711
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using THOC4/ALYREF Rabbit pAb (CAB6010) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using THOC4/THOC4/ALYREF Rabbit pAb (CAB6010) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of C6 cells using THOC4/ALYREF Rabbit pAb (CAB6010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using THOC4/ALYREF Rabbit pAb (CAB6010) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageConfocal immunofluorescence analysis of U2OS cells using THOC4/ALYREF Rabbit pAb (CAB6010) at dilution of 1:100. Blue: DAPI for nuclear staining.
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