Epigenetics & Nuclear Signaling Antibodies 2

Anti-ALKBH3 Antibody (CAB5808)

(No reviews yet) Write a Review
SKU:
CAB5808

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ALKBH3 Antibody
Antibody SKU:CAB5808
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human ALKBH3 (NP_631917.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Jurkat, 293T, HepG2, HeLa, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human ALKBH3 (NP_631917.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEEK RRRA RVQG AWAA PVKS QAIA QPAT TAKS HLHQ KPGQ TWKN KEHH LSDR EFVF KEPQ QVVR RAPE PRVI DREG VYEI SLSP TGVS RVCL YPGF VDVK EADW ILEQ LCQD VPWK QRTG IRED ITYQ QPRL TAWY GELP YTYS RITM EPNP HWHP VLRT LKNR IEEN TGHT FNSL LCNL YRNE KDSV DWHS DDEP SLGR CPII ASLS FGAT RTFE MRKK PPPE ENGD YTYV ERVK IPLD HGTL LIME GATQ ADWQ HRVP KEYH SREP RVNL TFRT VYPD PRGA PW
Gene ID:221120
Uniprot:Q96Q83
Cellular Location:Cytoplasm, Nucleus
Calculated MW:19kDa/33kDa
Observed MW:36kDa
Synonyms:ALKBH3, ABH3, DEPC-1, DEPC1, PCA1, hABH3
Background:
UniProt Protein Function:Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
NCBI Summary:The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
UniProt Code:Q96Q83
NCBI GenInfo Identifier:74752087
NCBI Gene ID:221120
NCBI Accession:Q96Q83.1
UniProt Secondary Accession:Q96Q83,Q3SYI0, Q6NX57, Q96BU8, A6NDJ1,
UniProt Related Accession:Q96Q83
Molecular Weight:19,310 Da
NCBI Full Name:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
NCBI Synonym Full Names:alkB homolog 3, alpha-ketoglutaratedependent dioxygenase
NCBI Official Symbol:ALKBH3  
NCBI Official Synonym Symbols:ABH3; PCA1; DEPC1; hABH3; DEPC-1  
NCBI Protein Information:alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
UniProt Protein Name:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
UniProt Synonym Protein Names:Alkylated DNA repair protein alkB homolog 3
UniProt Gene Name:ALKBH3  
Anti-ALKBH3 Antibody (CAB5808)Western blot analysis of extracts of various cell lines, using ALKBH3 antibody (CAB5808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
View AllClose

Additional Information

Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
View AllClose

Related Products