The TIFY11B Polyclonal Antibody (CAB21305) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is specifically designed for detecting TIFY11B in human samples using Western blot applications.TIFY11B is a protein that is involved in several important cellular processes, including immune response modulation and inflammatory signaling pathways. By targeting TIFY11B with this antibody, researchers can gain valuable insights into its function and its potential role in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
TIFY11B Polyclonal Antibody
SKU:
CAB21305
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
jasmonate-zim-domain protein 6, T10D10.8, T10D10_8, TIFY DOMAIN PROTEIN 11B, TIFY11B
Positive Sample:
Seedling, Inflorescences, Before inflorescence, Rosette leaf
Cellular Localization:
Cytoplasm.
Calculated MW:
30kDa
Observed MW:
25kDa
JAZ6 transcript levels rise in response to a jasmonate stimulus and a GFP:JAZ6 fusion protein localizes to the nucleus. Application of jasmonate methyl ester to Arabidopsis roots reduces the levels of a JAZ6:GUS fusion protein, presumably by stimulating ubiquitin-proteasome-mediated degradation.
Purification Method
Affinity purification
Gene ID
843577
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using TIFY11B Rabbit pAb (CAB21305) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
