Signaling Pathway Assays
Succinate Assay Kit (BA0149)
- SKU:
- BA0149
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Cells, Tissue
- Research Area:
- TCA Cycle
- Research Area:
- Diabetes & Obesity
- Research Area:
- Food Safety & Analysis
- Research Area:
- Plant & Environmental Stress
- Research Area:
- Clinical Chemistry
Description
About Succinate:
SUCCINATE, or succinic acid, can be found in all plants and animal tissues. It is an intermediate in the citric acid cycle and plays an important role in intracellular energy generation. Succinate is widely used as a flavoring agent in the food, beverage, and pharmaceutical industries due to its low toxicity.
How our Succinate Assay Kit Works:
Key Features
- Fast and sensitive. Use 20 uL sample. Linear detection range 10 to 400 uM for colorimetric assays and 2 to 40 uM for fluorimetric assays.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance or fluorescence after 30 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Succinate Assay Kit Information:
| Features | |
| Applications: | For quantitative determination of succinate (succinic acid) in food, beverage, agricultural products, and other biological samples. |
| Kit Includes: |
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| Kit Requires: | Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate or cuvette reader. |
| Method of Detection: |
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| Detection Limit: |
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| Samples: | Food, beverage, agricultural products, and other biological samples. |
| Species: | All |
| Protocol Length: | 30 min |
| Size: | 100 tests |
| Storage: | Store all components at -20°C upon receiving |
| Shelf Life: | 6 months |
Succinate Assay - Colorimetric Procedure:
| Step | Procedure |
| 1. | Internal standard is required for colorimetric assay. Each sample requires two separate reactions: 1) Sample plus internal standard and 2) Sample alone. In addition, each assay plate requires a water blank well. Add 20 µL of each sample to two separate wells. Also, add 20 µL dH2O to a separate well. For the internal standard, prepare 400 µL 1 mM succinate standard by mixing 20 µL 20 mM standard with 380 µL dH2O. Add 5 µL 1 mM standard to the sample plus internal standard wells. Add 5 µL dH2O to the sample alone and water wells. |
| 2. | Prepare sufficient Working Reagent (WR) for all wells by mixing, for each well, 85 µL Assay Buffer, 1 µL Enzyme Mix, 1 µL Cosubstrate, 1 µL PEP and 1 µL dye reagent. Fresh reconstitution of the WR is recommended. Add 80 µL WR to each well. Tap plate to mix. Incubate for 30 min at room temperature. |
| 3. | Read optical density at 570nm (550-585nm). |
Succinate Assay - Fluorimetric Procedure:
| Step | Procedure | ||||||||||||||||||||
| 1. | Prepare a 40 µM Standard Premix by mixing 20 µL of 1 mM succinate (see colorimetric internal standard procedure) with 480 µL dH2O. Dilute Standard in distilled water as follows: | ||||||||||||||||||||
Transfer 20 µL standards and 20 µL samples into separate wells of a black 96-well plate. | |||||||||||||||||||||
| 2. | Add 80 µL Working Reagent (see Colorimetric Procedure). Tap plate to mix. | ||||||||||||||||||||
| 3. | Incubate 30 min at room temperature and read fluorescence at ex/em = 530/585 nm. |
Related Products
Succinate Dehydrogenase (SDH) Activity Assay Kit
Human Succinate Dehydrogenase ELISA Kit
