Description
Formaldehyde Assay Kit (BA0247) (BA0247)
The Formaldehyde Assay Kit (SKU: BA0247) provides a rapid and sensitive colorimetric method for the quantitative determination of formaldehyde in biological samples, food, beverages and the environment. Formaldehyde (methanal) is the simplest aldehyde and is naturally present in all tissues and body fluids, with elevated urinary concentrations having been associated with certain cancers. The assay is based on formaldehyde dehydrogenase-catalysed oxidation of formaldehyde, generating formate and NADH which reduces a formazan (MTT) dye. The intensity of the reduced MTT, measured at 565 nm, is directly proportional to the formaldehyde concentration in the sample. Only 50 ยตL of sample is required, and the linear detection range spans 1.2 to 500 ยตM in a 96-well plate format. This convenient add-mix-read assay is readily automated for high-throughput screening of thousands of samples per day.
| Product Name: | Formaldehyde Assay Kit (BA0247) |
| SKU: | BA0247 |
| Detection Method: | Colorimetric (OD565nm) |
| Detection Range: | 1.2 to 500 ยตM |
| Sample Type: | Biological samples, food, beverages and the environment |
| Species Reactivity: | All |
| Assay Time: | 30 minutes |
| Kit Size: | 100 Assays |
| Equipment Required: | Microplate reader |
| Storage: | -20ยฐC |
| Shelf Life: | 6 months after receipt |
| Shipping: | Gel Pack |
A quantitative colorimetric assay for the determination of formaldehyde. The kit employs formaldehyde dehydrogenase-catalysed oxidation of formaldehyde to generate formate and NADH, which reduces an MTT formazan dye measured at 565 nm.
- Fast and sensitive. Use of 50 ยตL sample. Linear detection range 1.2 to 500 ยตM formaldehyde in a 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent and reading the absorbance after 30 minutes. Room temperature assay.
- High-throughput. Add-mix-read type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
- Direct assays of formaldehyde in biological samples, food, beverages and the environment
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Sample preparation: clear and slightly coloured samples can be assayed directly. Test several dilutions to determine an optimal dilution factor n. Biological fluids (e.g. urine and serum) can be assayed directly after centrifuging to remove particulates; appropriate dilution in distilled water may be required. |
| 2 | Standards: prepare 0.5 mL 500 ยตM Standard by mixing 25 ยตL Standard (10 mM) and 475 ยตL distilled water. Dilute standards in 1.5-mL centrifuge tubes as described in the standards table. |
| 3 | Transfer 50 ยตL standards into separate wells of a clear, flat-bottom 96-well plate. Transfer 50 ยตL of each sample into separate wells. |
| 4 | Prepare sufficient Working Reagent (WR) for all wells by mixing, for each well: 150 ยตL Assay Buffer, 10 ยตL MTT/NAD, 1 ยตL Enzyme A and 1 ยตL Enzyme B. Add 150 ยตL WR to the four standards and the sample wells. Tap the plate to mix briefly and thoroughly. Incubate 30 minutes at room temperature. |
| 5 | Read optical density at 565 nm (520-600 nm). |
Subtract the blank value (#4) from the standard values and plot the โOD against standard concentrations. Determine the slope and calculate the formaldehyde concentration of the sample: [Formaldehyde] = (ODSAMPLE โ ODBLANK) / Slope (ยตMโปยน) ร n (ยตM), where ODSAMPLE and ODBLANK are the optical density readings of the sample and water blank (#4) respectively, and n is the sample dilution factor. If the sample OD exceeds that of the 500 ยตM standard, dilute the sample in water and repeat the assay, multiplying the result by the dilution factor. Conversion: 1 ยตM formaldehyde equals 30 ppb.
| Component | Quantity | Storage |
| Assay Buffer | 15 mL | -20ยฐC |
| NAD/MTT | 1.0 mL | -20ยฐC |
| Enzyme A | 120 ยตL | -20ยฐC |
| Enzyme B | 120 ยตL | -20ยฐC |
| Standard (10 mM Formaldehyde) | 100 ยตL | -20ยฐC |
