Microbiology Recombinant Proteins
S. hygroscopicus Bar Recombinant Protein (RPES4076)
- SKU:
- RPES4076
- Product Type:
- Recombinant Protein
- Species:
- Streptomyces hygroscopicus
- Research Area:
- Microbiology
Description
S. hygroscopicus Bar Recombinant Protein
Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
| Product Name: | S. hygroscopicus Bar Recombinant Protein (RPES4076) |
| Product Code: | RPES4076 |
| Size: | 10µg |
| Species: | Streptomyces hygroscopicus |
| Expressed Host: | E.coli |
| Synonyms: | Phosphinothricin N-acetyltransferase, PPT N-acetyltransferase, Phosphinothricin-resistance protein, bar |
| Accession: | P16426 |
| Sequence: | Met1-Ile183 |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Streptomyces hygroscopicus Bar is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 20.6 kDa |
| AP Mol Mass: | 18-20 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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