RhoC Polyclonal Antibody (CAB21798)

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SKU:
CAB21798
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Applications:
IF
Reactivity:
Human, Mouse, Rat
Synonyms:
H9, ARH9, ARHC, RHOH9, RhoC
Frequently bought together:

Description

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RhoC Polyclonal Antibody (CAB21798)

The RhoC Polyclonal Antibody (CAB21798) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and is validated for Western blot applications. It detects the RHOC protein, allowing for detailed analysis in a variety of cell types.RhoC is a member of the Rho GTPase family, known for their roles in cytoskeletal dynamics and cell motility. Aberrant RhoC expression has been linked to tumor progression and metastasis in various cancers, making it an important target for therapeutic interventions.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RhoC Polyclonal Antibody
SKU:CAB21798
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:NIPE KWTP EVKH FCPN VPII LVGN KKDL RQDE HTRR ELAK MKQE PVRS EEGR DMAN RISA FGYL ECSA KTKE GVRE VFEM ATRA GLQV RKNK RRRG CPIL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:H9, ARH9, ARHC, RHOH9, RhoC
Positive Sample:Rat heart, A549
Cellular Localization:Cell Membrane, Cleavage Furrow, Cytoplasmic Side, Lipid-Anchor.
Calculated MW:22kDa
Observed MW:22kDa/21kDa

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Purification MethodAffinity purification
Gene ID389
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Rat heart, using RhoC Rabbit pAb (CAB21798) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of lysates from A549 cells using RhoC Rabbit pAb (CAB21798) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 90 s.
Product ImageImmunofluorescence analysis of PC-12 cells using RhoC Rabbit pAb (CAB21798) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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